Male or female between the ages of 18-40 years old.
Healthy and ready for physical activity as determined by questionnaires you complete about your health status.
Currently resistance training and have trained for a minimum of 6 months. To be included, you must have completed 3 resistance training sessions (defined as muscle strengthening activities such as free weights, weight machines, or calisthenics, etc.) per week with at least one lower body session. This will be determined by a Training History Questionnaire (THQ).
Premenopausal, with a regular menstrual period as determined by the menstrual status questionnaire (female participants only).
Currently not pregnant and do not intend to become pregnant (female participants
only).
Currently not taking dietary supplements that may affect study outcomes (e.g., creatine, beta-alanine) and willing refrain from taking such supplements while enrolled in the study. If you are currently using such supplements, you will be permitted to enroll in the study after not taking such supplements for a period of four weeks.
Willing to adhere to all pre-testing visit instructions including abstaining from exercise for the duration of the study and abstaining from alcohol for 24 hours prior to each study visit.
Currently consuming ≤ 300mg caffeine (≤ three 8oz cups of coffee) per day on average and willing to keep caffeine intake consistent throughout the duration of the study. Also willing to avoid caffeine intake for 12 hours prior to visits 3, 4 and 5 and not consume caffeine the morning of these visits.
Free from previous or current lower body injuries that may limit your ability to complete either exercise or physical assessments.
Highly likely to be successful at providing the required blood samples. This will be determined during visit 1 by a certified phlebotomist.
Not regularly taking any type of prescription or over-the-counter medication which might affect the assessments, or having any chronic illnesses, which require medical care. This will be determined in your medical history questionnaire.
